This project was designed to confirm the diagnosis of typhoid fever from stool culture of widal. The widal test is widely used in africa but little information exists about its reliability. Classically, a four fold rise of antibody in paired sera is considered. Introduction, principle, procedure, result interpretation, applications and limitations introduction. The result is interpreted with respect to the most dilute tube in the series that shows positive signs. The bacterium is spread due to intake of food or water contaminated with the faeces of an infected person poor hygienic conditions. In widal test, two types of tubes were originally used. Summary enteric fever occurs when pathogenic microorganisms like s. The test is named after georges fernand isidore widal, a french physician and bacteriologist, born march 9, 1862, algeria.
The test was developed by greembaum and widal in 1896. Widal test is a serological diagnosis test for enteric fever was founded by georges fernand isidore widal in 1896 sridhar rao, 2009 10. Sep 19, 2016 introduction of widal test widal test is a serological test that is done for the diagnosis of typhoid fever caused by salmonella organism. Diagnostic efficacy of widal slide agglutination test against widal. The widal test is an old serologic assay for detecting igm and igg antibodies to the o and h antigens of salmonella. Sep 17, 2014 based on the above results of widal test and blood culture for salmonella typhi and salmonella paratyphi, an evaluation of widal titration results for the diagnosis of typhoid fever was performed for o. Widal test is known to be one of the most reliable serological tests for enteric fever or undulant fever by bacteria causing typhoid and malaria where the serum is mixed with a specific antibody. It is advisable to inform the doctor about all the medicines and supplements that the patient is on, as certain medicines may. What are the values that should be considered normal and what values should be diagnostic of typhoid fever. The diagnostic ability of widal test is debatable as the test method has a low sensitivity, specificity and positive predictive value ppv.
It is conclude, that even today, the widal test remains one of the best, easily accessible, cheap and simple method for the diagnosis of typhoid fever. Understanding the widal test typhoid fever is a life threatening condition that attacks over 21 million people around the world each year. Over 100 years since its introduction as a serologic means of detecting the presence of typhoid fever, the widal test continues to be plagued with controversies involving the quality of the antigens used and interpretation of the result, particularly in endemic areas. Newer serologic assays are somewhat more sensitive and specific than the widal test, but are infrequently available. Hiper widal test teaching kit tube test is stable for 12 months from the date of manufacture without showing any reduction in performance. A comparative study of widal test with blood culture in the. The diagnostic value of an acutephase singletube widal test for suspected typhoid fever was evaluated with 2,000 vietnamese patients admitted to an infectious disease referral hospital between 1993 and 1998.
A comparative study of widal test with blood culture in. Add a drop of the antigen, which showed agglutination with the test sample in the screening qualitative method, to each. This test, derived from salmonella typhi o and h antigens, was performed in two ways. A slide widal test is, however, more popular among diagnostic laboratories as it provides rapid and immediate results. We assessed the performance of the widal tube agglutination test among febrile hospitalized tanzanian children. Agglutination reactions are routinely performed to type red blood cells. The weilfelix test can be done as either a slide or a tube test. Another approach is to incubate the tubes at 5055oc. What are the normal values for a widal test by agglutination methods. Diagnostic value of widal test in the diagnosis of typhoid. Widal test salmonella ab intended use spectrum diagnostics widal set is intended for the detection of antisalmonella antibodies in human serum. It helps to detect presence of salmonella antibodies in a patients serum. The widal test can be conducted via the two below listed methods. A similar study conducted in egypt indicates that a negative result of widal test would have a good predictive value of the disease npv 98% but positive result would have a very low predictive value for typhoid fever ppv 5.
During the course of disease, the body responds to this. The diagnostic value of an acutephase single tube widal test for suspected typhoid fever was evaluated with 2,000 vietnamese patients admitted to an infectious disease referral hospital between 1993 and 1998. Get your query answered 247 only on practo consult. Widal test is a common agglutination test employed in the serological diagnosis of enteric fever.
Widal test introduction, principle, procedure, interpretation and. Background ecteric fever occurs when pathogenic microorganisms like s. The widal test is one of the most overutilised diagnostic tests for typhoid fever in developing countries ever since its introduction over 100 years ago. The widal test was developed over a century ago and remains one of the worlds most widely used diagnostic tests but suffers from significant limitations in its sensitivity and specificity, as well as reliability. So, rapid and affordable diagnostic test like widal tube and slide agglutination test are used. Pdf clinical application of the widal test researchgate. Widal test is a tube agglutination test employed in the serological diagnosis of enteric fever. The tube agglutination test is a quantitative method which is used for the determination of titre values of antibodies present in the patient serum. The diagnosis of typhoid fever is confirmed by culture of salmonella enterica serotype typhi s. Widal test, introduction and procedure in lab youtube.
Although tube agglutination is the standard, some manufacturers have adapted it for slide agglutination. There is no denaturation of the antigen or the antibody during the reaction. Widal test interpretation, normal values, procedure. In typing for the abo antigens, rbcs are mixed on a slide with antisera to the a or b blood group antigens. The recommended method of performing the widal test is by the tube agglutination technique where serial twofold dilutions of the subjects serum from 1. Apr 14, 2020 tube agglutination method is the recommended method of performing the widal test. However, this requires equipment, supplies and trained laboratory personnel seldom found in primary healthcare facilities in the developing world. The slide test is rapid and is used as a screening procedure. Typhoid fever the common cause of morbidity and mortality especially in the developing countries where widal test is routinely used as diagnostic tool to rule out the disease. The widal tube agglutination test was done on all sera by the conventional agglutination method7 using commercially available antigens span diagnostic private limited.
After its development, the rapid slide test became the most commonly used technique in local laboratories because of its convenience. Widal test objective, principle, procedure, types, results. However no warranty or guarantee whatsoever is made or is to be implied with respect to such information or with respect to any product, method or apparatus referred to herein. The below table helps in understanding the widal weil felix test interpretation. The widal test is one method that may be used to help make a presumptive diagnosis of enteric fever, also known as typhoid fever. Salmonella typhi rod shaped, flagellated, aerobic, gram ve bacilli. On receipt, store the antigen solutions and test sample at 28 oc. A widal test helps in the rapid diagnosis of typhoid fever. Low sensitivity for widal test may also be related to the data collection time. Jan 28, 2017 widal test is done to diagnose typhoid fever. The widal test is a bacterial agglutination test which utilizes o and h antigens from enteric organisms. Also known as enteric fever, typhoid is rare in the united states and other developed countries but is still an ongoing threat to those who live or visit developing nations. Jun 22, 2010 the diagnosis of typhoid fever is confirmed by culture of salmonella enterica serotype typhi s.
Typhi from bone marrow is the current gold standard method for confirming a case of typhoid fever. Isolation of salmonella the causative agent from blood is the standard laboratory method for diagnosis, but it is not available at phc level. Widal test is a tube agglutination test employed in the serological diagnosis of. Value of a singletube widal test in diagnosis of typhoid. Enteric fever part 3 typhoid enteric fever diagnosis. The widal test has been used very extensively in the serodiagnosis of typhoid fever and, in developing countries, remains the only practical test available. Gillespie mb, bch, bao, mrcpuk, mrcpath, in medical microbiology illustrated, 1994. Widal tube test kit should be used according to the kit instructions. Introduction of widal test widal test is a serological test that is done for the diagnosis of typhoid fever caused by salmonella organism. Hiper widal test teaching kit tube test himedia laboratories.
Value of single widal the definitive diagnosis of typhoid. Who sensitivity and specificity of typhoid fever rapid. It talks about the tests principle, equipment, precautions, testing procedure and interpretation of results. The specificity however is not absolute and cross reactions may occur due to antigenic similarity or relatedness.
When running in the tube then incubate tubes for 12 hours or overnight. If the antigen is present on the cells, they agglutinate, forming a visible clump on the slide. It is advisable to inform the doctor about all the medicines and supplements that the patient is on, as certain medicines may affect the test results. Sensitivity and specificity of typhoid fever rapid. Typhoid fever is significant cause of morbidity across the. January 12, 2020 by sagar aryal widal test objective, principle, procedure, types, results, advantages and limitations. Prepare the serial dilution as shown in the diagram. Other problems with the widal test include the fact that it cannot distinguish between someone who currently has. A wide variety of widal test options are available to you. Enteric fever is the major health problem of developing country like india, with a notable morbidity and mortality. Widal test is still a common way of diagnosing enteric fever cases in several countries. Typhoid fever is significant cause of morbidity across the world, with an estimated.
We calculated the sensitivity, specificity, positive predictive value ppv, and negative predictive value npv of various antith and to titers using cultureconfirmed typhoid fever cases as the true positives and all other febrile children with blood culture negative. Comparative study of widal test against stool culture for typhoid fever suspected cases in southern ethiopia gemechu ameya,1 edemew atalel,2 berhanu kebede,3 bethel yohannes4 1department of medical laboratory science, college of medicine and health sciences, arba minch university, arba minch, 2department of medical laboratory science, durbetie primary. In 1896 and named after its inventor, georgesfernand widal, is a presumptive serological test for enteric fever or undulant fever whereby bacteria causing typhoid fever is mixed with a serum containing specific antibodies obtained from an infected individual. Tube agglutination method is the recommended method of performing the widal test.
The second kind of tests gives you more accurate results compared to the slide widal test. Enteric fever refers to typhoid and paratyphoid caused by salmonella typhi and salmonella paratyphi a, b, c respectively. Evaluation of the widal tube agglutination test for the diagnosis of typhoid fever among children admitted to a rural hospital in tanzania and a comparison with previous studies. There are many controversies regarding the widal test especially involving the quality of the antigens used and interpretation of the result, particularly in endemic areas. Get your query answered 247 with expert advice and. Pdf on jan 1, tshokey tshokey and others published interpretation of widal test a short presentation to stimulate a long discussion. This method is recommended for obtaining quick approximate titres only. It involves the use of a test tube or slide and a blood sample from the person suspected of having typhoid. Evaluation of the widal tube agglutination test for the.
Tube agglutination has more accuracy as compared to the slide agglutination technique. Two types of agglutination techniques are available. The antigens necessary ox2, ox19, and oxk can be obtained commercially. It may be noted that tube agglutination has more accuracy as compared to the slide agglutination technique. Test patients had suspected typhoid fever and a blood culture positive for salmonella typhi n 1,400 or salmonella paratyphi a n 45. Although the test is no longer commonly performed in the united states or other developed countries, it is still in use in many emerging nations where enteric fever is endemic and limited resources require the use of.
We assessed the performance of the widal tube agglutination test among febrile. This can be done as a slide method or as a tube method. It is an agglutination reaction that indicates the. Comparative study of widal test against stool culture for typhoid fever suspected cases in southern ethiopia gemechu ameya,1 edemew atalel,2 berhanu kebede,3 bethel yohannes4 1department of medical laboratory science, college of medicine and health sciences, arba minch university, arba minch, 2department of medical laboratory science, durbetie primary hospital, amahara, 3department of medical.
In cases of salmonella infection, it is a demonstration of. Widal test is used for the diagnosis of enteric fever. Widal test is an agglutination test which detects the presence of antibodies in patients serum produced against the causative agents of enteric fever salmonella typhi and salmonella paratyphi a, b and c. We calculated the sensitivity, specificity, positive predictive value ppv, and negative predictive value npv of various antith and to titers using cultureconfirmed typhoid fever cases as the true positives and all other febrile children with. About 19% of these are blood testing equipments, 31% are pathological analysis equipments, and % are clinical analytical instruments. Jan 12, 2020 widal test objective, principle, procedure, types, results, advantages and limitations.
Many studiesl5, however, have produced data which have cast serious doubts on the value of the widal test. Classic methods revisited widal agglutination test postgraduate. When facilities for culturing are not available, the widal test is the reliable and can be of value in the. There are a number of problems with widal agglutinations, most notably their low sensitivity and specificity. However, a slide widal test is more popular among diagnostic.
The recommended widal test is by tube agglutination method comments this test measures somatic o and flagellar h antibodies against typhoid and paratyphoid bacilli. Unfortunately, this method is slow and may take 714 days to confirm the diagnosis. Rock the slide gently back and forth, observe for agglutination macroscopically within one minute. Preparation for widal test slide agglutination test h suspension of bacteria is prepared by. Widal test objective, principle, procedure, types, results, advantages and limitations. Comparative study of widal test against stool culture for. However, a slide widal test is more popular among diagnostic laboratories as it gives rapid results. On a solid surface glass slide, tile, card, a small amount 50100. The test is unreliable, but is widely used in developing countries because of its low cost. Mar 29, 2011 widal test is still a common way of diagnosing enteric fever cases in several countries. The agglutinins usually appear at the end of the first week of infection and increase steadily till third fourth week after which the decline starts. This test detects the o and h antigen of salmonella typhi and paratyphi a,b and c. Widal test is a serological test which is used for the diagnosis of enteric fever or typhoid fever.
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