The diagnosis of typhoid fever is confirmed by culture of salmonella enterica serotype typhi s. The widal test is an old serologic assay for detecting igm and igg antibodies to the o and h antigens of salmonella. Widal test salmonella ab intended use spectrum diagnostics widal set is intended for the detection of antisalmonella antibodies in human serum. Classically, a four fold rise of antibody in paired sera is considered. Hiper widal test teaching kit tube test is stable for 12 months from the date of manufacture without showing any reduction in performance. The result is interpreted with respect to the most dilute tube in the series that shows positive signs. Test patients had suspected typhoid fever and a blood culture positive for salmonella typhi n 1,400 or salmonella paratyphi a n 45. Widal test is an agglutination test which detects the presence of antibodies in patients serum produced against the causative agents of enteric fever salmonella typhi and salmonella paratyphi a, b and c. This test, derived from salmonella typhi o and h antigens, was performed in two ways. The agglutinins usually appear at the end of the first week of infection and increase steadily till third fourth week after which the decline starts. Enteric fever part 3 typhoid enteric fever diagnosis. Although the test is no longer commonly performed in the united states or other developed countries, it is still in use in many emerging nations where enteric fever is endemic and limited resources require the use of. The diagnostic value of an acutephase single tube widal test for suspected typhoid fever was evaluated with 2,000 vietnamese patients admitted to an infectious disease referral hospital between 1993 and 1998. What are the normal values for a widal test by agglutination methods.
The test was developed by greembaum and widal in 1896. A similar study conducted in egypt indicates that a negative result of widal test would have a good predictive value of the disease npv 98% but positive result would have a very low predictive value for typhoid fever ppv 5. Enteric fever is the major health problem of developing country like india, with a notable morbidity and mortality. Over 100 years since its introduction as a serologic means of detecting the presence of typhoid fever, the widal test continues to be plagued with controversies involving the quality of the antigens used and interpretation of the result, particularly in endemic areas. So, rapid and affordable diagnostic test like widal tube and slide agglutination test are used. We assessed the performance of the widal tube agglutination test among febrile hospitalized tanzanian children. It is advisable to inform the doctor about all the medicines and supplements that the patient is on, as certain medicines may. Tube agglutination method is the recommended method of performing the widal test. Widal test interpretation, normal values, procedure. However, a more rapid, simpler, and cheaper diagnostic method would be very useful especially in developing countries. Typhoid fever is significant cause of morbidity across the.
Low sensitivity for widal test may also be related to the data collection time. The widal test is one method that may be used to help make a presumptive diagnosis of enteric fever, also known as typhoid fever. Hiper widal test teaching kit tube test himedia laboratories. Widal test is a tube agglutination test employed in the serological diagnosis of.
Evaluation of the widal tube agglutination test for the. In widal test, two types of tubes were originally used. Tube agglutination has more accuracy as compared to the slide agglutination technique. Unfortunately, this method is slow and may take 714 days to confirm the diagnosis.
The bacterium is spread due to intake of food or water contaminated with the faeces of an infected person poor hygienic conditions. Widal test is known to be one of the most reliable serological tests for enteric fever or undulant fever by bacteria causing typhoid and malaria where the serum is mixed with a specific antibody. The widal test is a bacterial agglutination test which utilizes o and h antigens from enteric organisms. Process of reading widal test report is quite comfortable where you will have to check for the highest dilution of the serum. The diagnostic value of an acutephase singletube widal test for suspected typhoid fever was evaluated with 2,000 vietnamese patients admitted to an infectious disease referral hospital between 1993 and 1998. The recommended method of performing the widal test is by the tube agglutination technique where serial twofold dilutions of the subjects serum from 1. There are many controversies regarding the widal test especially involving the quality of the antigens used and interpretation of the result, particularly in endemic areas.
Sep 17, 2014 based on the above results of widal test and blood culture for salmonella typhi and salmonella paratyphi, an evaluation of widal titration results for the diagnosis of typhoid fever was performed for o. The test is named after georges fernand isidore widal, a french physician and bacteriologist, born march 9, 1862, algeria. Widal test is still a common way of diagnosing enteric fever cases in several countries. When facilities for culturing are not available, the widal test is the reliable and can be of value in the. The test is unreliable, but is widely used in developing countries because of its low cost. The weilfelix test can be done as either a slide or a tube test. The tube agglutination test is a quantitative method which is used for the determination of titre values of antibodies present in the patient serum. The specificity however is not absolute and cross reactions may occur due to antigenic similarity or relatedness. Sep 19, 2016 introduction of widal test widal test is a serological test that is done for the diagnosis of typhoid fever caused by salmonella organism. The widal test can be conducted via the two below listed methods. This project was designed to confirm the diagnosis of typhoid fever from stool culture of widal.
Although tube agglutination is the standard, some manufacturers have adapted it for slide agglutination. What are the values that should be considered normal and what values should be diagnostic of typhoid fever. Many studiesl5, however, have produced data which have cast serious doubts on the value of the widal test. Jun 22, 2010 the diagnosis of typhoid fever is confirmed by culture of salmonella enterica serotype typhi s.
Agglutination reactions are routinely performed to type red blood cells. Widal test is a tube agglutination test employed in the serological diagnosis of enteric fever. It helps to detect presence of salmonella antibodies in a patients serum. It is an agglutination reaction that indicates the. This test detects the o and h antigen of salmonella typhi and paratyphi a,b and c. However, this requires equipment, supplies and trained laboratory personnel seldom found in primary healthcare facilities in the developing world. The slide test is rapid and is used as a screening procedure.
However, a slide widal test is more popular among diagnostic. On receipt, store the antigen solutions and test sample at 28 oc. Typhoid fever is significant cause of morbidity across the world, with an estimated. The antigens necessary ox2, ox19, and oxk can be obtained commercially. Typhoid fever the common cause of morbidity and mortality especially in the developing countries where widal test is routinely used as diagnostic tool to rule out the disease. Gillespie mb, bch, bao, mrcpuk, mrcpath, in medical microbiology illustrated, 1994. Widal tube test kit should be used according to the kit instructions. Widal test is a serological test which is used for the diagnosis of enteric fever or typhoid fever. Rock the slide gently back and forth, observe for agglutination macroscopically within one minute.
Jan 12, 2020 widal test objective, principle, procedure, types, results, advantages and limitations. Prepare the serial dilution as shown in the diagram. Enteric fever refers to typhoid and paratyphoid caused by salmonella typhi and salmonella paratyphi a, b, c respectively. Comparative study of widal test against stool culture for typhoid fever suspected cases in southern ethiopia gemechu ameya,1 edemew atalel,2 berhanu kebede,3 bethel yohannes4 1department of medical laboratory science, college of medicine and health sciences, arba minch university, arba minch, 2department of medical laboratory science, durbetie primary. A comparative study of widal test with blood culture in. It is conclude, that even today, the widal test remains one of the best, easily accessible, cheap and simple method for the diagnosis of typhoid fever. There are a number of problems with widal agglutinations, most notably their low sensitivity and specificity. Value of a singletube widal test in diagnosis of typhoid.
When running in the tube then incubate tubes for 12 hours or overnight. Introduction of widal test widal test is a serological test that is done for the diagnosis of typhoid fever caused by salmonella organism. Widal test is used for the diagnosis of enteric fever. If the antigen is present on the cells, they agglutinate, forming a visible clump on the slide. During the course of disease, the body responds to this. Widal test introduction, principle, procedure, interpretation and. However, a slide widal test is more popular among diagnostic laboratories as it gives rapid results. In typing for the abo antigens, rbcs are mixed on a slide with antisera to the a or b blood group antigens. Get your query answered 247 only on practo consult.
The widal test has been used very extensively in the serodiagnosis of typhoid fever and, in developing countries, remains the only practical test available. Widal test objective, principle, procedure, types, results, advantages and limitations. The recommended widal test is by tube agglutination method comments this test measures somatic o and flagellar h antibodies against typhoid and paratyphoid bacilli. Who sensitivity and specificity of typhoid fever rapid. A comparative study of widal test with blood culture in the. A wide variety of widal test options are available to you. Determination of which antigens are present on donor and recipient. Widal test is a common agglutination test employed in the serological diagnosis of enteric fever.
January 12, 2020 by sagar aryal widal test objective, principle, procedure, types, results, advantages and limitations. After its development, the rapid slide test became the most commonly used technique in local laboratories because of its convenience. This can be done as a slide method or as a tube method. Isolation of salmonella the causative agent from blood is the standard laboratory method for diagnosis, but it is not available at phc level. Pdf clinical application of the widal test researchgate. Typhi from bone marrow is the current gold standard method for confirming a case of typhoid fever. The second kind of tests gives you more accurate results compared to the slide widal test. Newer serologic assays are somewhat more sensitive and specific than the widal test, but are infrequently available. Evaluation of the widal tube agglutination test for the diagnosis of typhoid fever among children admitted to a rural hospital in tanzania and a comparison with previous studies. Sensitivity and specificity of typhoid fever rapid.
Get your query answered 247 with expert advice and. Apr 14, 2020 tube agglutination method is the recommended method of performing the widal test. Two types of agglutination techniques are available. Widal test objective, principle, procedure, types, results. Value of single widal the definitive diagnosis of typhoid. Salmonella typhi rod shaped, flagellated, aerobic, gram ve bacilli. In cases of salmonella infection, it is a demonstration of. Introduction, principle, procedure, result interpretation, applications and limitations introduction. The widal test is one of the most overutilised diagnostic tests for typhoid fever in developing countries ever since its introduction over 100 years ago. Background ecteric fever occurs when pathogenic microorganisms like s. Pdf on jan 1, tshokey tshokey and others published interpretation of widal test a short presentation to stimulate a long discussion. Other problems with the widal test include the fact that it cannot distinguish between someone who currently has. Widal test is a serological diagnosis test for enteric fever was founded by georges fernand isidore widal in 1896 sridhar rao, 2009 10. Summary enteric fever occurs when pathogenic microorganisms like s.
Preparation for widal test slide agglutination test h suspension of bacteria is prepared by. We calculated the sensitivity, specificity, positive predictive value ppv, and negative predictive value npv of various antith and to titers using cultureconfirmed typhoid fever cases as the true positives and all other febrile children with blood culture negative. Mar 29, 2011 widal test is still a common way of diagnosing enteric fever cases in several countries. There is no denaturation of the antigen or the antibody during the reaction. About 19% of these are blood testing equipments, 31% are pathological analysis equipments, and % are clinical analytical instruments.
Jan 28, 2017 widal test is done to diagnose typhoid fever. However no warranty or guarantee whatsoever is made or is to be implied with respect to such information or with respect to any product, method or apparatus referred to herein. Add a drop of the antigen, which showed agglutination with the test sample in the screening qualitative method, to each. Another approach is to incubate the tubes at 5055oc. A widal test helps in the rapid diagnosis of typhoid fever. Diagnostic efficacy of widal slide agglutination test against widal. Also known as enteric fever, typhoid is rare in the united states and other developed countries but is still an ongoing threat to those who live or visit developing nations. This method is recommended for obtaining quick approximate titres only. It talks about the tests principle, equipment, precautions, testing procedure and interpretation of results. It is advisable to inform the doctor about all the medicines and supplements that the patient is on, as certain medicines may affect the test results. The widal test is widely used in africa but little information exists about its reliability.
Diagnostic value of widal test in the diagnosis of typhoid. In 1896 and named after its inventor, georgesfernand widal, is a presumptive serological test for enteric fever or undulant fever whereby bacteria causing typhoid fever is mixed with a serum containing specific antibodies obtained from an infected individual. The below table helps in understanding the widal weil felix test interpretation. Comparative study of widal test against stool culture for typhoid fever suspected cases in southern ethiopia gemechu ameya,1 edemew atalel,2 berhanu kebede,3 bethel yohannes4 1department of medical laboratory science, college of medicine and health sciences, arba minch university, arba minch, 2department of medical laboratory science, durbetie primary hospital, amahara, 3department of medical. It involves the use of a test tube or slide and a blood sample from the person suspected of having typhoid. A slide widal test is, however, more popular among diagnostic laboratories as it provides rapid and immediate results. We assessed the performance of the widal tube agglutination test among febrile. Comparative study of widal test against stool culture for. Classic methods revisited widal agglutination test postgraduate. The widal tube agglutination test was done on all sera by the conventional agglutination method7 using commercially available antigens span diagnostic private limited. Understanding the widal test typhoid fever is a life threatening condition that attacks over 21 million people around the world each year.
1018 655 1196 180 693 868 1608 1035 256 734 96 1521 333 953 548 317 176 539 809 1617 8 1156 526 1226 1477 598 672 1193 877 717 10 641 92 314 1202 202 1040 548 501 703 294